Measurement of peroxiredoxin activity.
نویسندگان
چکیده
Peroxiredoxins are cysteine-dependent peroxidases that react with hydrogen peroxide, larger hydroperoxide substrates, and peroxynitrite. Protocols are provided to measure Prx activity with peroxide by (1) a coupled reaction with NADPH, thioredoxin reductase, and thioredoxin, (2) the direct monitoring of thioredoxin oxidation, (3) competition with horseradish peroxidase, and (4) peroxide consumption using the FOX assay.
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ورودعنوان ژورنال:
- Current protocols in toxicology
دوره Chapter 7 شماره
صفحات -
تاریخ انتشار 2011